ISO 21675:2019 Method

Our solutions for assessing water quality and monitoring persistent pollutants.

To fully support the application of ISO 21675:2019, Wellington has developed solutions and mixtures of native and labelled PFAS, specifically designed for this method.

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Our Solutions/Mixtures developed for the ISO 21675:2019 method

ISO 21675-NSS and ISO 21675-LSS mixtures from Wellington Laboratories are certified standards designed specifically for the application of ISO 21675:2019, used for the analysis of PFAS (per- and polyfluoroalkylated substances) in water.

The combined use of these two mixtures ensures that the ISO 21675:2019 method is applied with maximum analytical rigour.

All our mixtures are available as 1.2 mL solutions in methanol.

Native PFAS standards, targeted analytes in ISO 21675 method.

PFAS-labelled standards, targeted analytes in ISO 21675 method.

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High precision

These innovative solutions enable reliable identification and quantification of target compounds thanks to their exceptional purity and optimum compatibility with analytical protocols.

Designed to meet the rigorous demands of laboratories, our mixtures offer unrivalled analytical performance, ensuring reproducible results that comply with the most stringent regulatory standards. With Wellington, you benefit from reference tools for carrying out cutting-edge environmental analyses.

Certified reliability

Each batch is accompanied by a detailed certificate of analysis, attesting to the quality and purity of the compounds.

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France and Belgium.

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ISO 21675-NSS mixture

ISO 21675:2019 Native Stock Solution

This mixture contains isotopically unlabelled standards of PFAS; analytes targeted in the ISO 21675 method.

    • Composition: a panel of native PFAS substances commonly detected in the environment, such as PFOA, PFOS, PFHxA, PFNA, etc.
    • Example of application: to establish the calibration curve and quantify the analytes.

List of compounds

CompoundAcronymConcentration
(ng/mL)
Perfluoro-n-butanoic acidPFBA100
Perfluoro-n-pentanoic acidPFPeA100
Perfluoro-n-hexanoic acidPFHxA100
Perfluoro-n-heptanoic acidPFHpA100
Perfluoro-n-octanoic acidPFOA100
Perfluoro-n-nonanoic acidPFNA100
Perfluoro-n-decanoic acidPFDA100
Perfluoro-n-undecanoic acidPFUdA100
Perfluoro-n-dodecanoic acidPFDoA100
Perfluoro-n-tridecanoic acidPFTrDA100
Perfluoro-n-tetradecanoic acidPFTeDA100
Perfluoro-n-hexadecanoic acidPFHxDA100
Perfluoro-n-octadecanoic acidPFODA100
Perfluoro-1-octanesulfonamideFOSA100
N-Methylperfluoro-1-octanesulfonamideN-MeFOSA100
N-Ethylperfluoro-1-octanesulfonamideN-EtFOSA100
N-Methylperfluoro-1-octanesulfonamidoacetic acidN-MeFOSAA100
N-Ethylperfluoro-1-octanesulfonamidoacetic acidN-EtFOSAA100
2H-Perfluoro-2-decenoic acidFOUEA100
2,3,3,3-Tetrafluoro-2-(1,1,2,2,3,3,3-heptafluoropropoxy)propanoic acidHFPO-DA100
Potassium perfluoro-1-butanesulfonatePFBS100*
Sodium perfluoro-1-hexanesulfonatePFHxS100*
Sodium perfluoro-1-heptanesulfonatePFHpS100*
Sodium perfluoro-1-octanesulfonatePFOS100*
Sodium perfluoro-1-decanesulfonatePFDS100*
Sodium 1H,1H,2H,2H-perfluorooctanesulfonate6:2FTS100*
Sodium 1H,1H,2H,2H-perfluorodecanesulfonate8:2FTS100*
Sodium dodecafluoro-3H-4,8-dioxanonanoateNaDONA100*
Potassium 9-chlorohexadecafluoro-3-oxanonane-1-sulfonate9Cl-PF3ONS100*
Sodium bis(1H,1H,2H,2H-perfluorodecyl) phosphate8:2diPAP100*

*The concentration indicated is reported in salt form.

ISO 21675-LSS mixture

ISO 21675:2019 Labelled Stock Solution

This mixture contains isotopically labelled standards of PFAS, the analytes targeted in the ISO 21675 method.

    • Composition: a range of PFAS identical to those in the NSS mixture, but isotopically labelled.
    • Example of application: as internal references to correct bias, guaranteeing robust and reliable results.

List of compounds

CompoundAcronymConcentration
(ng/mL)
Perfluoro-n-(1,2,3,4-13C4)butanoic acidMPFBA100
Perfluoro-n-(1,2,3,4,5-13C5)pentanoic acidM5PFPeA100
Perfluoro-n-(1,2,3,4,6-13C5)hexanoic acidM5PFHxA100
Perfluoro-n-(1,2,3,4-13C4)heptanoic acidM4PFHpA100
Perfluoro-n-(13C8)octanoic acidM8PFOA100
Perfluoro-n-(13C9)nonanoic acidM9PFNA100
Perfluoro-n-(1,2,3,4,5,6-13C6)decanoic acidM6PFDA100
Perfluoro-n-(1,2,3,4,5,6,7-13C7)undecanoic acidM7PFUdA100
Perfluoro-n-(1,2-13C2)dodecanoic acidMPFDoA100
Perfluoro-n-(1,2-13C2)tetradecanoic acidM2PFTeDA100
Perfluoro-n-(1,2-13C2)hexadecanoic acidM2PFHxDA100
Perfluoro-1-(13C8)octanesulfonamideM8FOSA100
N-methyl-d3-perfluoro-1-octanesulfonamided-N-MeFOSA100
N-ethyl-d5-perfluoro-1-octanesulfonamided-N-EtFOSA100
N-methyl-d3-perfluoro-1-octanesulfonamidoacetic acidd3-N-MeFOSAA100
N-ethyl-d5-perfluoro-1-octanesulfonamidoacetic acidd5-N-EtFOSAA100
2H-Perfluoro-(1,2-13C2)-2-decenoic acid (8:2)MFOUEA100
2,3,3,3-Tetrafluoro-2-(1,1,2,2,3,3,3-heptafluoropropoxy)(13C3)propanoic acidM3HFPO-DA100
Sodium perfluoro-1-(2,3,4-13C3)butanesulfonateM3PFBS100*
Sodium perfluoro-1-(1,2,3-13C3)hexanesulfonateM3PFHxS100*
Sodium perfluoro-(13C8)octanesulfonateM8PFOS100*
Sodium 1H,1H,2H,2H-perfluoro(1,2-13C2)octanesulfonate (6:2)M2-6:2FTS100*
Sodium 1H,1H,2H,2H-perfluoro(1,2-13C2)decanesulfonate (8:2)M2-8:2FTS100*
Sodium bis[1H,1H,2H,2H-(1,2-13C2)perfluorodecyl] phosphateM4-8:2diPAP100*

*The concentration indicated is reported in salt form.

Wellington Laboratories reagent categories

PFAS, per- polyfluoroalkyl Substances

Halogenated flame retardants, HFRs and other compounds

Polychlorinated biphenyls, PCBs

Polybrominated diphenyl ethers, PBDEs Polybrominated biphenyls, PBBs

Dioxins, Furans, PCDDs, PCDFs

Organochlorine Pesticides, OCP

Certified reference materials

Other reagents

Find out more about the ISO 21675 standard

ISO 21675:2019 specifies a method for the determination of per- and polyfluoroalkylated substances (PFAS) in water, including drinking water, groundwater and surface water. It uses liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) to detect and quantify these contaminants. This method is essential for assessing water quality and monitoring persistent pollutants.

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