EPA-1633 Method

Detect and quantify PFAS in compliance with EPA-1633.

To fully support the application of EPA-1633, Wellington has developed solutions and mixtures of native and labelled PFAS, specifically designed for this method.

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Our Solutions/Mixtures for EPA-1633 Method

These mixtures, such as EPA-1633STK, contain quantitative isomeric mixtures of PFAS that were not previously available on the market. They can be diluted and/or combined with other mixtures prepared by Wellington Laboratories to obtain the spiking and calibration solutions recommended by the method: MPFAC-HIF-ES and MPFAC-HIF-ES.

The use of these mixtures ensures greater precision and reliability in the analysis of PFAS analysis, making it easier to implement EPA-1633 in a variety of environmental contexts.

Thanks to these innovations, laboratories can now carry out more complete and accurate analyses , contributing to better management of the environmental risks associated with PFAS.

All our mixtures are available as 1.2 mL solutions in methanol.

Native PFAS standards, analytes targeted in EPA method 1633

Extraction mixture of PFAS-labelled standards

Injection mixture of 5 PFAS-labelled standards

Our commitments

High precision

These innovative solutions enable reliable identification and quantification of target compounds thanks to their exceptional purity and optimum compatibility with analytical protocols.

Designed to meet the rigorous demands of laboratories, our mixtures offer unrivalled analytical performance, ensuring reproducible results that comply with the most stringent regulatory standards. With Wellington, you benefit from reference tools for carrying out cutting-edge environmental analyses.

Certified reliability

Each batch is accompanied by a detailed certificate of analysis, attesting to the quality and purity of the compounds.

Fast delivery

France and Belgium.

Do you have a question? Contact us using the contact form. We will be delighted to advise you.

EPA-1633STK Mixture

EPA Method 1633 Native PFAS Stock Solution/Mixture

Surrogate standards are used to monitor the quality of analyses, and the EPA-1633STK fulfils this function by providing reliable internal references for the entire analytical process. These standards are essential for laboratories following the EPA-1633 method, which requires a high level of precision and traceability in the analysis of PFAS.

    • Type of mixture: Surrogate Standard solution.
    • Composition: EPA-1633STK is a solution/mixture of native perfluoroalkylcarboxylicacids (linear and branched), perfluorinated alkanesulphonates (linear and branched), fluorotelomer sulphonates (4:2, 6:2, and 8:2), perfluorooctanesulfonamides (linear and branched), perfluorooctanesulfonamidoacetic acids (linear and branched), perfluorooctanesulfonamidoethanols (linear and branched), hexafluoropropylene oxide dimer acid (GenX, HFPO-DA), sodium dodecafluoro-3H-4, 8- dioxanonanoate (NaDONA), perfluoroalkyl ether carboxylic acids (PF4OPeA, PF50HxA and 3,6-OPFHpA), the major and minor components of F-53B (9CI-PF3ONS and 11CI-PF3OUdS), a perfluoroalkyl ether sulphonate (PFEESA) and fluorotelomer carboxylic acids.
    • Example of application: added to samples (water, soil, biosolids) before processing to monitor analytical recovery. It can also be used to detect potential losses during the preparation or analysis stages.

List of compounds

CompoundAcronymConcentration (ng/mL)
Perfluoro-n-butanoic acidPFBA1000
Perfluoro-n-pentanoic acidPFPeA500
Perfluoro-n-hexanoic acidPFHxA250
Perfluoro-n-heptanoic acidPFHpA250
Perfluorooctanoic acid*PFOA250*
Perfluorononanoic acid*PFNA250*
Perfluoro-n-decanoic acidPFDA250
Perfluoro-n-undecanoic acidPFUdA250
Perfluoro-n-dodecanoic acidPFDoA250
Perfluoro-n-tridecanoic acidPFTrDA250
Perfluoro-n-tetradecanoic acidPFTeDA250
Perfluorooctanesulfonamide*FOSA250*
N-Methylperfluorooctanesulfonamide*N-MeFOSA250*
N-Ethylperfluorooctanesulfonamide*N-EtFOSA250*
N-Methylperfluorooctanesulfonamidoacetic acid*N-MeFOSAA250*
N-Ethylperfluorooctanesulfonamidoacetic acid*N-EtFOSAA250*
2-(N-Methylperfluorooctanesulfonamido)ethanol*N-MeFOSE250*
2-(N-Ethylperfluorooctanesulfonamido)ethanol*N-EtFOSE250*
2,3,3,3-Tetrafluoro-2-(1,1,2,2,3,3,3-heptafluoropropoxy)propanoic acidHFPO-DA1000
Perfluoro-4-oxapentanoic acidPF4OPeA500
Perfluoro-5-oxahexanoic acidPF5OHxA500
Perfluoro-3,6-dioxaheptanoic acid3,6-OPFHpA500
3-Perfluoropropyl propanoic acidFPrPA1250
3-Perfluoropentyl propanoic acidFPePA6250
3-Perfluoroheptyl propanoic acidFHpPA6250
Potassium perfluoro-1-butanesulfonateL-PFBS250
Sodium perfluoro-1-pentanesulfonateL-PFPeS250
Potassium perfluorohexanesulfonate*PFHxSK250*
Sodium perfluoro-1-heptanesulfonateL-PFHpS250
Potassium perfluorooctanesulfonate*PFOSK250*
Sodium perfluoro-1-nonanesulfonateL-PFNS250
Sodium perfluoro-1-decanesulfonateL-PFDS250
Sodium perfluoro-1-dodecanesulfonateL-PFDoS250
Sodium 1H,1H,2H,2H-perfluorohexanesulfonate4:2FTS1000
Sodium 1H,1H,2H,2H-perfluorooctanesulfonate6:2FTS1000
Sodium 1H,1H,2H,2H-perfluorodecanesulfonate8:2FTS1000
Sodium dodecafluoro-3H-4,8-dioxanonanoateNaDONA1000
Potassium 9-chlorohexadecafluoro-3-oxanonane-1-sulfonate9Cl-PF3ONS1000
Potassium 11-chloroeicosafluoro-3-oxaundecane-1-sulfonate11Cl-PF3OUdS1000
Potassium perfluoro(2-ethoxyethane)sulfonatePFEESA500

* Linear and branched isomers. The concentration indicated is reported in salt form.

MPFAC-HIF-ES Mixture

Mass-Labelled PFAS Extraction Standard Solution/Mixture

    • Type of mixture: solution of PFAS standards with isotopes of carbon 13 (13C) or deuterium (2H).
    • Composition: MPFAC-HIF-ES is a solution/mixture of (13C) -labelled perfluoroalkylcarboxylicacids (C4-C12, C14), ( 13C) -labelled perfluoroalkanesulphonates (C4, C6 and C8), ( 13C) -labelled perfluoro-1-octanesulphonamides (one 13C and two 2H), ( 13C) -labelled fluorotelomer sulphonates ( 4:2, 6:2 and 8:2) , labelled perfluoro-1-octanesulfonamidoacetic acids (2H), labelled perfluoro-1-octanesulfonamidoethanols ( 2H) and hexafluoropropylene oxide dimer acid (13C3-GenX, M3HFPO-DA).
    • Example of application: added before extraction to compensate for losses and standardise results when analysing complex matrices (water, soils, biosolids, etc.).

List of compounds

CompoundAcronymConcentration (ng/mL)
Perfluoro-n-(1,2,3,4-13C4)butanoic acidMPFBA2000
Perfluoro-n-(1,2,3,4,5-13C5)pentanoic acidM5PFPeA1000
Perfluoro-n-(1,2,3,4,6-13C5)hexanoic acidM5PFHxA500
Perfluoro-n-(1,2,3,4-13C4)heptanoic acidM4PFHpA500
Perfluoro-n-(13C8)octanoic acidM8PFOA500
Perfluoro-n-(13C9)nonanoic acidM9PFNA250
Perfluoro-n-(1,2,3,4,5,6-13C6)decanoic acidM6PFDA250
Perfluoro-n-(1,2,3,4,5,6,7-13C7)undecanoic acidM7PFUdA250
Perfluoro-n-(1,2-13C2)dodecanoic acidMPFDoA250
Perfluoro-n-(1,2-13C2)tetradecanoic acidM2PFTeDA250
Perfluoro-1-(13C8)octanesulfonamideM8FOSA500
N-methyl-d3-perfluoro-1-octanesulfonamided-N-MeFOSA500
N-ethyl-d5-perfluoro-1-octanesulfonamided-N-EtFOSA500
N-methyl-d3-perfluoro-1-octanesulfonamidoacetic acidd3-N-MeFOSAA1000
N-ethyl-d5-perfluoro-1-octanesulfonamidoacetic acidd5-N-EtFOSAA1000
2-(N-methyl-d3-perfluoro-1-octanesulfonamido)ethan-d4-old7-N-MeFOSE5000
2-(N-ethyl-d5-perfluoro-1-octanesulfonamido)ethan-d4-old9-N-EtFOSE5000
2,3,3,3-Tetrafluoro-2-(1,1,2,2,3,3,3-heptafluoropropoxy)(13C3)propanoic acidM3HFPO-DA2000
Sodium perfluoro-1-(2,3,4-13C3)butanesulfonateM3PFBS500*
Sodium perfluoro-1-(1,2,3-13C3)hexanesulfonateM3PFHxS500*
Sodium perfluoro-(13C8)octanesulfonateM8PFOS500*
Sodium 1H,1H,2H,2H-perfluoro(1,2-13C2)hexanesulfonate (4:2)M2-4:2FTS1000*
Sodium 1H,1H,2H,2H-perfluoro(1,2-13C2)octanesulfonate (6:2)M2-6:2FTS1000*
Sodium 1H,1H,2H,2H-perfluoro(1,2-13C2)decanesulfonate (8:2)M2-8:2FTS1000*

*The concentration indicated is reported in salt form.

MPFAC-HIF-IS Mixture

Mass-Labelled PFAS Injection Standard Solution/Mixture

    • Type of mixture: solution of PFAS standards with carbon 13 (13C) isotopes.
    • Composition: MPFAC-HIF-IS is a solution/mixture of 5 PFCAs (C4, C6, C8-C10) labelled (13C) and 2 perfluoroalkanesulphonates PFSAs (C6 and C8) labelled (18O and 13C).
    • Example of application: precise quantification of targeted PFCAs and PFSAs .

List of compounds

CompoundAcronymConcentration (ng/mL)
Perfluoro-n-(2,3,4-13C3)butanoic acidM3PFBA1000
Perfluoro-n-(1,2-13C2)hexanoic acidMPFHxA500
Perfluoro-n-(1,2,3,4-13C4)octanoic acidMPFOA500
Perfluoro-n-(1,2,3,4,5-13C5)nonanoic acidMPFNA250
Perfluoro-n-(1,2-13C2)decanoic acidMPFDA250
Sodium perfluoro-1-hexane(18O2)sulfonateMPFHxS500*
Sodium perfluoro-1-(1,2,3,4-13C4)octanesulfonateMPFOS500*

*The concentration indicated is reported in salt form.

EPA 1633 SOLUTION/MIXTURES

Catalogue No.DescriptionUnitSolvent
EPA-1633STKEPA Method 1633 Native PFAS Stock Solution/Mixture1.2 mLmethanol
MPFAC-HIF-ESMass-Labelled PFAS Extraction Standard Solution/Mixture 1.2 mLmethanol
MPFAC-HIF-ISMass-Labelled PFAS Injection Standard Solution/Mixture 1.2 mLmethanol

Do you have a question? Contact us using the contact form. We will be delighted to advise you.

Wellington Laboratories reagent categories

PFAS, per- polyfluoroalkyl Substances

Halogenated flame retardants, HFRs and other compounds

Polychlorinated biphenyls, PCBs

Polybrominated diphenyl ethers, PBDEs Polybrominated biphenyls, PBBs

Dioxins, Furans, PCDDs, PCDFs

Organochlorine Pesticides, OCP

Certified reference materials

Other reagents

Find out more about EPA-1633 Method

EPA Method 1633 is a standardised analytical procedure for detecting and quantifying PFAS in non-drinking water matrices. Developed by the US Environmental Protection Agency (EPA), this method uses liquid chromatography coupled with mass spectrometry (LC-MS/MS). It can identify and measure up to 40 different PFAS compounds. This method is therefore essential for monitoring PFAS in various non-drinking water matrices, helping to assess the risks associated with these persistent contaminants.

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