EPA-537.1 Analytical Method

Detect and quantify PFAS with mixtures certified and prepared by Wellington for EPA Method 537.1.

To fully support the application of EPA-537.1, Wellington has developed solutions and mixtures of native and labelled PFAS, specifically designed for this method.

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Our mixtures are developed in accordance with EPA Method 537.1

These mixtures are designed to meet the analytical requirements of the method, improving the accuracy and sensitivity of the measurements.

All our mixtures are available as 1.2 mL solutions in methanol.

Solution of isotopically mass-labelled substitution standards

Mass-labelled isotope standards for accurate quantification by LC-MS/MS

Basic native standards for the calibration of target analytes

Extended native standards, covering a wider range of PFASs

Additional native standards to complete PFAS analysis

Our commitments

High precision

These innovative solutions enable reliable identification and quantification of target compounds thanks to their exceptional purity and optimum compatibility with analytical protocols.

Designed to meet the rigorous demands of laboratories, our mixtures offer unrivalled analytical performance, ensuring reproducible results that comply with the most stringent regulatory standards. With Wellington, you benefit from reference tools for carrying out cutting-edge environmental analyses.

Certified reliability

Each batch is accompanied by a detailed certificate of analysis, attesting to the quality and purity of the compounds.

Fast delivery

France and Belgium.

Do you have a question? Contact us using the contact form. We will be delighted to advise you.

EPA-537ss-R1 mixture

EPA Method 537 Surrogate Primary Dilution Standard (SUR PDS)

The EPA-537SS-R1 mixture is a solution of isotopically-labelled substitution standards used for the analysis of PFAS in drinking water according to EPA method 537.1. These standards are used as internal references to correct analytical variations and guarantee accurate quantification of the target analytes using liquid chromatography coupled with mass spectrometry (LC-MS/MS).

List of compounds

CompoundAcronymConcentration
(ng/mL)
Perfluoro-n-(1,2-13C2)hexanoic acidMPFHxA1000
Perfluoro-n-(1,2-13C2)decanoic acidMPFDA1000
2,3,3,3-Tetrafluoro-2-(1,1,2,2,3,3,3-heptafluoropropoxy)(13C3)propanoic acidM3HFPO-DA1000
N-ethyl-d5-perfluoro-1-octanesulfonamidoacetic acidd5-N-EtFOSAA4000

EPA-537IS mixture

EPA Method 537 Internal Standard Primary Dilution Standard (IS PDS)

The EPA-537IS mixture developed by Wellington is a solution of isotopic standards used for the analysis of per- and polyfluoroalkylated substances (PFAS) in drinking water, in accordance with EPA method 537. It contains labelled isotopes of specific PFAS compounds, enabling accurate and reliable quantification by liquid chromatography-mass spectrometry (LC-MS/MS).

List of compounds

CompoundAcronymConcentration
(ng/mL)
Perfluoro-n-(1,2-13C2)octanoic acidM2PFOA1000
N-methyl-d3-perfluoro-1-octanesulfonamidoacetic acidd3-N-MeFOSAA4000
Sodium perfluoro-1-(1,2,3,4-13C4)octanesulfonateMPFOS3000*

*The concentration indicated is reported in salt form.

EPA-537PDS-R1 mixture

EPA Method 537 Analyte Primary Dilution Standard (branched/linear mix)

The EPA-537PDS-R1 mixture prepared by Wellington is a solution of native standards designed for the analysis of per- and polyfluoroalkylated substances (PFAS) in drinking water according to EPA method 537. It contains unlabelled PFAS, used as standards for the calibration and quantification of target analytes in liquid chromatography coupled to mass spectrometry (LC-MS/MS).

List of compounds

CompoundAcronymConcentration (ng/mL)
Perfluoro-n-hexanoic acidPFHxA2000
Perfluoro-n-heptanoic acidPFHpA2000
Perfluoro-n-octanoic acidPFOA2000
Perfluoro-n-nonanoic acidPFNA2000
Perfluoro-n-decanoic acidPFDA2000
Perfluoro-n-undecanoic acidPFUdA2000
Perfluoro-n-dodecanoic acidPFDoA2000
Perfluoro-n-tridecanoic acidPFTrDA2000
Perfluoro-n-tetradecanoic acidPFTeDA2000
2,3,3,3-Tetrafluoro-2-(1,1,2,2,3,3,3-heptafluoropropoxy)propanoic acidHFPO-DA2000
N-Methylperfluoro-1-octanesulfonamidoacetic acidN-MeFOSAA: linear isomer
N-MeFOSAA: ∑ branched isomers
1520
480
N-Ethylperfluoro-1-octanesulfonamidoacetic acidN-EtFOSAA: linear isomer
N-EtFOSAA: ∑ branched isomers
1550
450
Potassium perfluoro-1-butanesulfonateL-PFBS2000*
Potassium perfluorohexanesulfonate^{c}PFHxSK: linear isomer
PFHxSK: ∑ branched isomers
1620*
378*
Potassium perfluorooctanesulfonate^{d}PFOSK: linear isomer
PFOSK: ∑ branched isomers
1460*
392*
Sodium dodecafluoro-3H-4,8-dioxanonanoateNaDONA2000*
Potassium 9-chlorohexadecafluoro-3-oxanonane-1-sulfonate9Cl-PF3ONS2000*
Potassium 11-chloroeicosafluoro-3-oxaundecane-1-sulfonate11Cl-PF3OUdS2000*

*The concentration indicated is reported in salt form.

EPA-537PDSL-R1 mixture

EPA Method 537 Analyte Primary Dilution Standard (linear isomers only)

Wellington’s EPA-537PDSL-R1 mixture is an extended native standards solution for the analysis of per- and polyfluoroalkyl substances (PFAS) in drinking water in accordance with EPA Method 537. It contains an extended panel of unlabelled PFAS, allowing wider analytical coverage for environmental studies by LC-MS/MS.

List of compounds

CompoundAcronymConcentration (ng/mL)
Perfluoro-n-hexanoic acidPFHxA2000
Perfluoro-n-heptanoic acidPFHpA2000
Perfluoro-n-octanoic acidPFOA2000
Perfluoro-n-nonanoic acidPFNA2000
Perfluoro-n-decanoic acidPFDA2000
Perfluoro-n-undecanoic acidPFUdA2000
Perfluoro-n-dodecanoic acidPFDoA2000
Perfluoro-n-tridecanoic acidPFTrDA2000
Perfluoro-n-tetradecanoic acidPFTeDA2000
2,3,3,3-Tetrafluoro-2-(1,1,2,2,3,3,3-heptafluoropropoxy)propanoic acidHFPO-DA2000
N-Methylperfluoro-1-octanesulfonamidoacetic acidN-MeFOSAA2000
N-Ethylperfluoro-1-octanesulfonamidoacetic acidN-EtFOSAA2000
Potassium perfluoro-1-butanesulfonateL-PFBS2000*
Sodium perfluoro-1-hexanesulfonateL-PFHxS2000*
Sodium perfluoro-1-octanesulfonateL-PFOS2000*
Sodium dodecafluoro-3H-4,8-dioxanonanoateNaDONA2000*
Potassium 9-chlorohexadecafluoro-3-oxanonane-1-sulfonate9Cl-PF3ONS2000*
Potassium 11-chloroeicosafluoro-3-oxaundecane-1-sulfonate11Cl-PF3OUdS2000*

*The concentration indicated is reported in salt form.

EPA-537APDS mixture

EPA Method 537 Native Analyte Primary Dilution Standard (expanded br/l mix)

Wellington’s EPA-537APDS mixture is a solution of additional native standards designed to complement the analysis of PFAS in drinking water using EPA Method 537. It includes additional PFAS compounds not covered by the base mixtures, thus extending the detection and quantification capabilities of LC-MS/MS.

List of compounds

CompoundAcronymConcentration (ng/mL)
Perfluoro-n-hexanoic acidPFHxA2000 ng/mL
Perfluoro-n-heptanoic acidPFHpA2000 ng/mL
Perfluorooctanoic acid (linear and branched isomers)br-PFOA2000 ng/mL
Perfluorononanoic acid (linear and branched isomers)br-PFNA2000 ng/mL
Perfluoro-n-decanoic acidPFDA2000 ng/mL
Perfluoro-n-undecanoic acidPFUdA2000 ng/mL
Perfluoro-n-dodecanoic acidPFDoA2000 ng/mL
Perfluoro-n-tridecanoic acidPFTrDA2000 ng/mL
Perfluoro-n-tetradecanoic acidPFTeDA2000 ng/mL
2,3,3,3-Tetrafluoro-2-(1,1,2,2,3,3,3-heptafluoroproproxy)propanoic acidHFPO-DA2000 ng/mL
N-Methylperfluorooctanesulfonamidoacetic acid (linear and branched isomers)br-NMeFOSAA2000 ng/mL
N-Ethylperfluorooctanesulfonamidoacetic acid (linear and branched isomers)br-NEtFOSAA2000 ng/mL
Potassium perfluoro-1-butanesulfonateL-PFBS2000 ng/mL*
Potassium perfluorohexanesulfonate (linear and branched isomers)br-PFHxSK2000 ng/mL*
Potassium perfluorooctanesulfonate (linear and branched isomers)br-PFOSK2000 ng/mL*
Sodium dodecafluoro-3H-4,8-dioxanonanoateNaDONA2000 ng/mL*
Potassium 9-chlorohexadecafluoro-3-oxanonane-1-sulfonate9Cl-PF3ONS2000 ng/mL*
Potassium 11-chloroeicosafluoro-3-oxaundecane-1-sulfonate11Cl-PF3OUdS2000 ng/mL*

*The concentration indicated is reported in salt form.

EPA-537.1 SOLUTION/MIXTURES

Catalogue No.DescriptionUnitSolvent
EPA-537SS-R1EPA Method 537 Surrogate Primary Dilution Standard (SUR PDS)1.2 mLmethanol
EPA-537ISEPA Method 537 Internal Standard Primary Dilution Standard (IS PDS)1.2 mLmethanol
EPA-537PDS-R1EPA Method 537 Analyte Primary Dilution Standard (branched/linear mix)1.2 mLmethanol
EPA-537PDSL-R1EPA Method 537 Analyte Primary Dilution Standard (linear isomers only)1.2 mLmethanol
EPA-537APDSEPA Method 537 Native Analyte Primary Dilution Standard (expanded br/l mix)1.2 mLmethanol

Do you have a question? Contact us using the contact form. We will be delighted to advise you.

Wellington Laboratories reagent categories

PFAS, per- polyfluoroalkyl Substances

Halogenated flame retardants, HFRs and other compounds

Polychlorinated biphenyls, PCBs

Polybrominated diphenyl ethers, PBDEs Polybrominated biphenyls, PBBs

Dioxins, Furans, PCDDs, PCDFs

Organochlorine Pesticides, OCP

Certified reference materials

Other reagents

Find out more about EPA-537.1 Analytical Method

EPA 537 Method is an analytical procedure developed by the US Environmental Protection Agency (EPA) for the detection and quantification of per- and polyfluoroalkyl compounds (PFAS) in drinking water. It uses liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) to accurately analyse these contaminants at trace levels.

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