EPA-533 Method
Our analytical standards for assessing water quality and monitoring persistent pollutants.To guarantee compliant and accurate analyses in accordance with EPA-533, we offer a complete range of native and labelled PFAS standards.
This range is specifically designed to support laboratories in the accurate quantification of analytes in complex matrices, the creation of reliable calibration curves, and the effective correction of analytical biases, guaranteeing results of unrivalled accuracy.
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Our mixtures developed for EPA Method 533
Wellington mixtures are concentrated solutions of analytical standards, carefully developed and certified to offer exceptional quality and reliability. Designed specifically for use with EPA-533, these mixtures meet the requirements of environmental analysis laboratories.
All our mixtures are available as 1.2 mL solutions in methanol.
High-performance PDS mix: 16 13C-labelled standards
PDS mixture of 3 PFAS-labelled standards, analytes targeted in EPA method 533
25 native analytes, ideal for preparing accurate and reproducible calibration solutions
25 native standards, with PFOA, PFNA, PFHxS and PFOS in their linear and branched forms
Our commitments
High precision
These innovative solutions enable reliable identification and quantification of target compounds thanks to their exceptional purity and optimum compatibility with analytical protocols.
Designed to meet the rigorous demands of laboratories, our mixtures offer unrivalled analytical performance, ensuring reproducible results that comply with the most stringent regulatory standards. With Wellington, you benefit from reference tools for carrying out cutting-edge environmental analyses.
Certified reliability
Each batch is accompanied by a detailed certificate of analysis, attesting to the quality and purity of the compounds.
Fast delivery
France and Belgium.
Do you have a question? Contact us using the contact form. We will be delighted to advise you.
EPA-533ES
EPA Method 533 Isotope Dilution Standard PDS
EPA-533ES is a high-performance PDS mixture containing 16 isotopically-labelled standards; analytes targeted in EPA method 533. Ideal for the quantification of per- and polyfluoroalkyl compounds (PFAS) in drinking water samples.
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- Mixture type: EPA-533ES is a Primary Dilution Standard (PDS) containing stable isotopic compounds labelled with carbon 13 (13C). It is used as an internal standard for LC-MS/MS analyses in accordance with the requirements of EPA method 533.
- Composition: contains 16 isotopically labelled compounds, selected to correspond directly to the native analytes targeted by the EPA 533 method. These compounds include 9 labelled perfluorocarboxylic acids (PFCAs) (PFBA, PFPeA, PFHxA and PFOA), 3 labelled perfluorosulphonic acids (PFSAs) (PFBS, PFHxS, and PFOS), 3 labelled sulphonated fluorotelomers (FTS) (4:2, 6:2 and 8:2) and 1 labelled hexafluoropropylene oxide dimer acid (GenX) (HFPO-DA).
- Example of application: designed for quantification by isotopic dilution, which is a reference method recognised for its accuracy and reliability.
List of compounds
| Compound | Acronym | Concentration (ng/mL) |
|---|---|---|
| Perfluoro-n-(1,2,3,4-13C4)butanoic acid | MPFBA | 500 |
| Perfluoro-n-(1,2,3,4,5-13C5)pentanoic acid | M5PFPeA | 500 |
| Perfluoro-n-(1,2,3,4,6-13C5)hexanoic acid | M5PFHxA | 500 |
| Perfluoro-n-(1,2,3,4-13C4)heptanoic acid | M4PFHpA | 500 |
| Perfluoro-n-(13C8)octanoic acid | M8PFOA | 500 |
| Perfluoro-n-(13C9)nonanoic acid | M9PFNA | 500 |
| Perfluoro-n-(1,2,3,4,5,6-13C6)decanoic acid | M6PFDA | 500 |
| Perfluoro-n-(1,2,3,4,5,6,7-13C7)undecanoic acid | M7PFUdA | 500 |
| Perfluoro-n-(1,2-13C2)dodecanoic acid | MPFDoA | 500 |
| 2,3,3,3-Tetrafluoro-2-(1,1,2,2,3,3,3-heptafluoropropoxy)(13C3)propanoic acid | M3HFPO-DA | 500 |
| Sodium perfluoro-1-(2,3,4-13C3)butanesulfonate | M3PFBS | 500* |
| Sodium perfluoro-1-(1,2,3-13C3)hexanesulfonate | M3PFHxS | 500* |
| Sodium perfluoro-(13C8)octanesulfonate | M8PFOS | 500* |
| Sodium 1H,1H,2H,2H-perfluoro(1,2-13C2)hexanesulfonate (4:2) | M2-4:2FTS | 2000* |
| Sodium 1H,1H,2H,2H-perfluoro(1,2-13C2)octanesulfonate (6:2) | M2-6:2FTS | 2000* |
| Sodium 1H,1H,2H,2H-perfluoro(1,2-13C2)decanesulfonate (8:2) | M2-8:2FTS | 2000* |
*The concentration indicated is reported in salt form.
EPA-533IS
EPA Method 533 Isotope Performance Standard PDS
This mixture contains isotopically labelled standards of PFAS; analytes targeted in EPA method 533.
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- Type of mixture: EPA-533IS is a Primary Dilution Standard (PDS), made up of labelled stable isotopic compounds, used as internal standards in LC-MS/MS analyses. These isotopic standards make it possible to improve the reliability and reproducibility of measurements, taking into account instrumental variations and analytical losses.
- Composition: this mixture contains homologs of the native analytes targeted by EPA method 533. It is composed of 2 13C labelled perfluorocarboxylic acids (PFCAs) (M3PFBA and M2PFOA) and 1 13C-labelled perfluorosulphonic acid (PFSAs) (MPFOS).
- Example of application: used as an internal standard to standardise analyses by correcting for analytical bias and any losses.
List of compounds
| Compound | Acronym | Concentration (ng/mL) |
|---|---|---|
| Perfluoro-n-(2,3,4-13C3)butanoic acid | M3PFBA | 1000 |
| Perfluoro-n-(1,2-13C2)octanoic acid | M2PFOA | 1000 |
| Sodium perfluoro-1-(1,2,3,4-13C4)octanesulfonate | MPFOS | 3000* |
*The concentration indicated is reported in salt form.
EPA-533PAR
EPA Method 533 Native Analyte Primary Dilution Standard (br/linear mix)
The mixture includes a selection of native PFAS representative of the analytes targeted by EPA Method 533, in their linear and branched forms.
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- Mixture type: EPA-533PAR is a Primary Dilution Standard (PDS) containing only native analytes. It is designed for analytical applications requiring the accurate quantification of native PFAS, taking into account their structural isomers (branched and linear).
- Composition: includes native linear perfluoroalkylcarboxylic acids (PFCAs),
linear and branched perfluoroalkanesulfonates (PFSAs), 3 fluorotelomer sulfonates (FTS: 4:2, 6:2 and 8:2), sodium dodecafluoro-3H-4,8-dioxanonanoate (NaDONA), 2 major and minor components of F-53B (9Cl-PF3ONS and 11Cl-PF3OUdS), 1 hexafluoropropylene oxide dimer acid (GenX, HFPO-DA), 3 perfluoroalkyl ether carboxylic acids (PF4OPeA, PF5OHxA and 3,6-OPFHpA), 1 perfluoroalkyl ether sulphonate (PFEESA). - Example of application: for the detection and quantification of native PFAS in their linear and branched forms in drinking water samples, in accordance with EPA method 533.
List of compounds
| Compound | Acronym | Concentration (ng/mL) |
|---|---|---|
| Perfluoro-n-butanoic acid | PFBA | 500 |
| Perfluoro-n-pentanoic acid | PFPeA | 500 |
| Perfluoro-n-hexanoic acid | PFHxA | 500 |
| Perfluoro-n-heptanoic acid | PFHpA | 500 |
| Perfluoro-n-octanoic acid | PFOA | 500 |
| Perfluoro-n-nonanoic acid | PFNA | 500 |
| Perfluoro-n-decanoic acid | PFDA | 500 |
| Perfluoro-n-undecanoic acid | PFUdA | 500 |
| Perfluoro-n-dodecanoic acid | PFDoA | 500 |
| 2,3,3,3-Tetrafluoro-2-(1,1,2,2,3,3,3-heptafluoropropoxy)propanoic acid | HFPO-DA | 500 |
| Perfluoro-4-oxapentanoic acid | PF4OPeA | 500 |
| Perfluoro-5-oxahexanoic acid | PF5OHxA | 500 |
| Perfluoro-3,6-dioxaheptanoic acid | 3,6-OPFHpA | 500 |
| Potassium perfluoro-1-butanesulfonate | L-PFBS | 500* |
| Sodium perfluoro-1-pentanesulfonate | L-PFPeS | 500* |
| Potassium perfluorohexanesulfonate | PFHxSK: linear isomer PFHxSK: ∑ branched isomers | 406* 94* |
| Sodium perfluoro-1-heptanesulfonate | L-PFHpS | 500* |
| Potassium perfluorooctanesulfonate | PFOSK: linear isomer PFOSK: ∑ branched isomers | 394* 106* |
| Sodium 1H,1H,2H,2H-perfluoro-1-hexanesulfonate | 4:2FTS | 500* |
| Sodium 1H,1H,2H,2H-perfluoro-1-octanesulfonate | 6:2FTS | 500* |
| Sodium 1H,1H,2H,2H-perfluoro-1-decanesulfonate | 8:2FTS | 500* |
| Sodium dodecafluoro-3H-4,8-dioxanonanoate | NaDONA | 500* |
| Potassium 9-chlorohexadecafluoro-3-oxanonane-1-sulfonate | 9Cl-PF3ONS | 500* |
| Potassium 11-chloroeicosafluoro-3-oxaundecane-1-sulfonate | 11Cl-PF3OUdS | 500* |
| Potassium perfluoro(2-ethoxyethane)sulfonate | PFEESA | 500* |
*The concentration indicated is reported in salt form.
EPA-533APDS
EPA Method 533 Native Analyte Primary
Dilution Standard (expanded br/l mix)
This mixture is the most complete in the range. It contains native PFAS standards, the analytes targeted in EPA method 533.
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- Mixture type: EPA-533APDS is a Primary Dilution Standard (PDS) containing certified native standards. It is intended for dilution and calibration for analysis in accordance with EPA method 533.
- Composition: includes linear and branched perfluoroalkylcarboxylic acids (PFCAs),
linear and branched perfluoroalkanesulfonates (PFSAs), 3 fluorotelomer sulfonates (FTS: 4:2, 6:2 and 8:2), sodium dodecafluoro-3H-4,8-dioxanonanoate (NaDONA), 2 major and minor components of F-53B (9Cl-PF3ONS and 11Cl-PF3OUdS), 1 hexafluoropropylene oxide dimer acid (GenX, HFPO-DA), 3 perfluoroalkyl ether carboxylic acids (PF4OPeA, PF5OHxA and 3,6-OPFHpA), 1 perfluoroalkyl ether sulphonate (PFEESA). - Example of application: to check analytical performance. It can be used to validate laboratory methods, ensuring that detection and quantification limits comply with the thresholds set by the EPA.
List of compounds
| Compound | Acronym | Concentration (ng/mL) | |
|---|---|---|---|
| Perfluoro-n-butanoic acid | PFBA | 500 | |
| Perfluoro-n-pentanoic acid | PFPeA | 500 | |
| Perfluoro-n-hexanoic acid | PFHxA | 500 | |
| Perfluoro-n-heptanoic acid | PFHpA | 500 | |
| Perfluorooctanoic acid | PFOA: linear isomer PFOA: ∑ branched isomers | 331 169 | |
| Perfluorononanoic acid | PFNA: linear isomer PFNA: ∑ branched isomers | 326 174 | |
| Perfluoro-n-decanoic acid | PFDA | 500 | |
| Perfluoro-n-undecanoic acid | PFUdA | 500 | |
| Perfluoro-n-dodecanoic acid | PFDoA | 500 | |
| 2,3,3,3-Tetrafluoro-2-(1,1,2,2,3,3,3-heptafluoropropoxy)propanoic acid | HFPO-DA | 500 | |
| Perfluoro-4-oxapentanoic acid | PF4OPeA | 500 | |
| Perfluoro-5-oxahexanoic acid | PF5OHxA | 500 | |
| Perfluoro-3,6-dioxaheptanoic acid | 3,6-OPFHpA | 500 | |
| Potassium perfluoro-1-butanesulfonate | L-PFBS | 500* | |
| Sodium perfluoro-1-pentanesulfonate | L-PFPeS | 500* | |
| Potassium perfluorohexanesulfonate | PFHxSK: linear isomer PFHxSK: ∑ branched isomers | 406* 94* | |
| Sodium perfluoro-1-heptanesulfonate | L-PFHpS | 500* | |
| Potassium perfluorooctanesulfonate | PFOSK: linear isomer PFOSK: ∑ branched isomers | 394* 106* | |
| Sodium 1H,1H,2H,2H-perfluoro-1-hexanesulfonate | 4:2FTS | 500* | |
| Sodium 1H,1H,2H,2H-perfluoro-1-octanesulfonate | 6:2FTS | 500* | |
| Sodium 1H,1H,2H,2H-perfluoro-1-decanesulfonate | 8:2FTS | 500* | |
| Sodium dodecafluoro-3H-4,8-dioxanonanoate | NaDONA | 500* | |
| Potassium 9-chlorohexadecafluoro-3-oxanonane-1-sulfonate | 9Cl-PF3ONS | 500* | |
| Potassium 11-chloroeicosafluoro-3-oxaundecane-1-sulfonate | 11Cl-PF3OUdS | 500* | |
| Potassium perfluoro(2-ethoxyethane)sulfonate | PFEESA | 500* |
*The concentration indicated is reported in salt form.
EPA-533 SOLUTION/MIXTURES
| Catalogue No. | Description | Unit | Solvent |
|---|---|---|---|
| EPA-533ES | EPA Method 533 Isotope Dilution Standard PDS | 1.2 mL | methanol |
| EPA-533IS | EPA Method 533 Isotope Performance Standard PDS | 1.2 mL | methanol |
| EPA-533PAR | EPA Method 533 Native Analyte Primary Dilution Standard (br/linear mix) | 1.2 mL | methanol |
| EPA-533APDS | EPA Method 533 Native Analyte Primary Dilution Standard (expanded br/l mix) | 1.2 mL | methanol |
Do you have a question? Contact us using the contact form. We will be delighted to advise you.
PFAS, per- polyfluoroalkyl Substances
Halogenated flame retardants, HFRs and other compounds
Polychlorinated biphenyls, PCBs
Polybrominated diphenyl ethers, PBDEs Polybrominated biphenyls, PBBs
Dioxins, Furans, PCDDs, PCDFs
Organochlorine Pesticides, OCP
Certified reference materials
Other reagents
Find out more about EPA-533 Method
EPA method 533 is a standardised analytical procedure for detecting and quantifying PFAS in drinking water matrices. Developed by the US Environmental Protection Agency (EPA), the method is based on liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). EPA method 533 measures 25 precise PFAS (none with more than 12 carbon atoms), including perfluorinated acids, sulphonates, fluorotelomer sulphonates and poly- and perfluorinated ether carboxylic acids. It is used in particular by environmental laboratories to monitor water quality and ensure compliance with safety standards.
In addition, this method complements method 537.1, since it incorporates 14 of the 18 PFAS covered by method 537.1. Method 533 measures 11 more PFAS than method 537.1. This includes more hydrophilic chemicals such as PFBA. By combining the two methods, 29 different PFAS can be measured in drinking water.
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